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Elispot for Rookies (and Experts Too)

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This book provides basic, simple, and logical explanations for choices to be made to run the best Elispot possible. It allows the newcomer to truly understand the best options for specific protocol steps, reagents and materials, and provides even the experienced Elispot user with insight into best practices. The techniques presented here are supported by the author’s twenty-plus years of first-hand experience working with this assay, as well as the shared experiences of numerous colleagues and collaborators.

The enzyme-linked immunospot (Elispot) assay is a widely used technique to monitor cells on the single cell level for the release of analytes like cytokines, chemokines or immunoglobulins (antibodies), in response to particular stimuli. The most important feature of Elispot is its outstanding sensitivity, allowing the detection of specific cells in very low frequencies. The advantages of Elispot have resulted in it being widely adapted for use in research and translational applications in numerous fields including cancer, infectious diseases, autoimmunity, and transplantation.

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Detail Information

Series Title

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Call Number

-

Publisher

: ., 2016

Collation

7 b/w illustrations, 32 illustrations in colour

Language

English

ISBN/ISSN

978-3-319-45295-1

Classification

NONE

Content Type

text

Media Type

computer

Carrier Type

online resource

Edition

1

Subject(s)

Cell Biology
Biomedical Research
Immunology

Specific Detail Info

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Statement of Responsibility

Sylvia Janetzki

Other Information

Cataloger

agus

Source

-

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