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Advanced Photon Counting: Applications, Methods, Instrumentation

This volume focuses on Time-Correlated Single Photon Counting (TCSPC), a powerful tool allowing luminescence lifetime measurements to be made with high temporal resolution, even on single molecules. Combining spectrum and lifetime provides a “fingerprint” for identifying such molecules in the presence of a background. Used together with confocal detection, this permits single-molecule spectroscopy and microscopy in addition to ensemble measurements, opening up an enormous range of hot life science applications such as fluorescence lifetime imaging (FLIM) and measurement of Förster Resonant Energy Transfer (FRET) for the investigation of protein folding and interaction. Several technology-related chapters present both the basics and current state-of-the-art, in particular of TCSPC electronics, photon detectors and lasers. The remaining chapters cover a broad range of applications and methodologies for experiments and data analysis, including the life sciences, defect centers in diamonds, super-resolution microscopy, and optical tomography. The chapters detailing new options arising from the combination of classic TCSPC and fluorescence lifetime with methods based on intensity fluctuation represent a particularly unique highlight.

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Detail Information
Series Title
Springer Series on Fluorescence
Call Number
621.366 ADV a
Publisher
Cham, Switzerland : Springer.,
Collation
XII, 370
Language
English
ISBN/ISSN
978-3-319-15636-1
Classification
621.366
Content Type
text
Media Type
computer
Carrier Type
online resource
Edition
Ed. 1
Subject(s)
Biophysics
Spectroscopy
Laser
Specific Detail Info
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Statement of Responsibility
Other Information
Cataloger
umi
Source
https://link.springer.com/content/pdf/10.1007/978-3-319-15636-1.pdf?pdf=button
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