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Efficient Screening Techniques to Identify Mutants with TR4 Resistance in Banana

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Mutagenesis of in vitro propagated bananas is an efficient method to
introduce novel alleles and broaden genetic diversity. Mutations can be induced by
treatment of plant cells with chemical mutagens or ionizing radiation. The
FAO/IAEA Plant Breeding and Genetics Laboratory established efficient methods
for mutation induction of in vitro shoot tips in banana using physical and chemical
mutagens as well as methods for the efficient discovery of EMS-induced single
nucleotide mutations in targeted genes or amplicons and identification of large
genomic changes, including deletions and insertions. Mutagenesis of in vitro propagated tissues requires large populations serving as starting material, and a long
process to dissolve genetic mosaics (chimeras) resulting from the mutagenesis of
multicellular tissues. However, treating shoot apical meristems of tissue cultured
bananas with a mutagen is a commonly used practice for banana mutation breeding
programmes, and still the most effective. In our previous studies, we showed that
chimeras, unique mutations accumulated in different cells of the plant propagule,
could be rapidly removed via isolation of shoot apical meristems and subsequent
longitudinal bisection

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Publisher

: Springer Berlin, Heidelberg., 2022

Collation

XVI, 187

Language

English

ISBN/ISSN

9783662649152

Classification

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Content Type

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Media Type

computer

Carrier Type

online resource

Edition

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Subject(s)

Efficient Screening Techniques
Identify Mutants with TR4 Resistance in Banana

Specific Detail Info

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Statement of Responsibility

Joanna Jankowicz-Cieslak, Ivan L. Ingelbrecht

Other Information

Cataloger

angga

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Digital Object Identifier (DOI)

https://link.springer.com/book/10.1007/978-3-662-64915-2

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