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Biocatalytic Synthesis of Bioactive Compounds
8-Hydroxydaidzein (8-OHDe), an ortho-hydroxylation derivative of soy isoflavone
daidzein isolated from some fermented soybean foods, has been demonstrated to possess potent
anti-inflammatory activity. However, the isoflavone aglycone is poorly soluble and unstable in
alkaline solutions. To improve the aqueous solubility and stability of the functional isoflavone,
8-OHDe was glucosylated with recombinant amylosucrase of Deinococcus geothermalis (DgAS) with
industrial sucrose, instead of expensive uridine diphosphate-glucose (UDP-glucose
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