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Advances in Biotechnology Volume 1

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The Results of Our Investigation on the Microbial Community of the High-Temperature Dagang Oilfield (P.r. China) Are Summarized. Detailed Experimental Data Are Provided on Syntrophic Acetate Degradation by Thermophilic Associations, on the Isolation of Pure Cultures From These Associations, Their Physiologicalcharacteristics, and Reconstruction of Microbial Interactions During Acetate Degradation to Methane. The Microbial Community of the High-Temperature Dagang Oilfield Was Investigated by Culture-Based, Radioisotope, and 16s Rrna Gene Techniques. Cultivable Microorganisms (Aerobic Oil-Oxidizing, Anaerobic Fermentative, Sulfate-Reducing, and Methanogenic) Were Found in Formation Water. Methanogenic Enrichments Were Obtained in Media Both With н2 +CO2 and Acetate. The Process of Methane Production in Formation Waters Was Also Registered by Radioisotope Methods With na2 14co3 and 14ch3 Coona. However, Pure Cultures of Thermophilic Aceticlastic Methanogens Were Not Obtained. The 16s Rrna Gene Analysis of the Formation Water and Methanogenic Enrichments Revealed That h2 -Utilizing Methanogens of the Genus Methanothermobacter Were Predominant in the Archaeal Libraries (97% Of Archaeal
Clones). Phylotypes of Acetate-Utilizing Methanogens Were Detected in the Libraries Only After the Acetate Content in Formation Water Was Increased. Bacterial Phylotypes
Belonged to the Known Fermentative (Thermoanaerobacter, Thermoterrabacterium, Thermovenabulum, Fervidobacterium, Thermotoga, Dictyoglomus, Pedobacter, and Dysgonomonas), Sulfate-Reducing (Thermodesulfovibrio and Desulfotomaculum), and Syntrophic (Thermacetogenium and Thermovirga) Bacteria. Pure Cultures of Methanothermobacter SP. And Thermoanaerobacter Ethanolicus Were Isolated From Thermophilic Methanogenic Enrichments Grown on Acetate. The Binary Culture Combining Both Strains Carried Out the Reaction of Syntrophic Acetate Degradation to Methane. The Data Obtained Show That Syntrophic Associations May Contribute Significantly to Methane Production in High-Temperature Petroleum Reservoirs. One of Important Ecological Function of Fermentative Bacteria of the Thermoanaerobacter– Caldanaerobacter Group Is Their Ability to Carry Out Syntrophic Acetate Oxidation to Н2
and со2 , Which Is Then Reduced to Methane by Hydrogenothrophic Methanogens.

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Series Title

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Call Number

630

Publisher

: ., March 25, 2022

Collation

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Language

English

ISBN/ISSN

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Classification

630

Content Type

text

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Edition

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Subject(s)

Biotechnology

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Statement of Responsibility

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Other Information

Cataloger

Suwardi

Source

https://docs.google.com/viewerng/viewer?url=http://uilis.unsyiah.ac.id/oer/files/original/61f1baf2f7e0ba0a0e95da67bacca591.pdf

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